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rabbit anti cd86 polyclonal antibody  (Cell Signaling Technology Inc)


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    Cell Signaling Technology Inc rabbit anti cd86 polyclonal antibody
    Pro-inflammatory milieu in calcified arteries and the impact of guide wires with different diameters on MAC model generation. (A) Representative IF staining results showed that CD68 (green color) and <t>CD86</t> (red color) expression was low in the mice from the sham operation group and significantly higher in the mice from the experimental group in terms of the fluorescence density and intensity. Individual channels are presented separately for clarity. Nuclei were counterstained with DAPI (not shown). (B) Representative IHC staining results showed that the IL-1β and IL-6 expression in the calcified blood vessel from the experimental group was significantly higher than that in the sham operation group, with slightly higher intensity. (C) Incidence rates of ossification caused by the 0.35, 0.40, and 0.45 mm diameter guide wires. (D) According to the MAC histopathological grading criteria , the MAC grading ratios for the MAC caused by the 0.35, 0.40, and 0.45 mm diameter guide wires in the mouse MAC. Sample sizes were: n=10 for Sham group, n=12 for 0.35 mm group, and n=13 for 0.40 mm group, n=15 for 0.45 mm group. (E) Image of rough guide wires with diameters of 0.35, 0.40, or 0.45 mm. DAPI, 4',6-diamidino-2-phenylindole; IF, immunofluorescent; IHC, immunohistochemistry; IL-1β, Interleukin-1 beta; IL-6, Interleukin-6; MAC, medial arterial calcification.
    Rabbit Anti Cd86 Polyclonal Antibody, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 97/100, based on 228 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/rabbit anti cd86 polyclonal antibody/product/Cell Signaling Technology Inc
    Average 97 stars, based on 228 article reviews
    rabbit anti cd86 polyclonal antibody - by Bioz Stars, 2026-05
    97/100 stars

    Images

    1) Product Images from "Establishment of a medial arterial calcification model in C57BL/6J mice via arterial intimal injury"

    Article Title: Establishment of a medial arterial calcification model in C57BL/6J mice via arterial intimal injury

    Journal: Cardiovascular Diagnosis and Therapy

    doi: 10.21037/cdt-2025-435

    Pro-inflammatory milieu in calcified arteries and the impact of guide wires with different diameters on MAC model generation. (A) Representative IF staining results showed that CD68 (green color) and CD86 (red color) expression was low in the mice from the sham operation group and significantly higher in the mice from the experimental group in terms of the fluorescence density and intensity. Individual channels are presented separately for clarity. Nuclei were counterstained with DAPI (not shown). (B) Representative IHC staining results showed that the IL-1β and IL-6 expression in the calcified blood vessel from the experimental group was significantly higher than that in the sham operation group, with slightly higher intensity. (C) Incidence rates of ossification caused by the 0.35, 0.40, and 0.45 mm diameter guide wires. (D) According to the MAC histopathological grading criteria , the MAC grading ratios for the MAC caused by the 0.35, 0.40, and 0.45 mm diameter guide wires in the mouse MAC. Sample sizes were: n=10 for Sham group, n=12 for 0.35 mm group, and n=13 for 0.40 mm group, n=15 for 0.45 mm group. (E) Image of rough guide wires with diameters of 0.35, 0.40, or 0.45 mm. DAPI, 4',6-diamidino-2-phenylindole; IF, immunofluorescent; IHC, immunohistochemistry; IL-1β, Interleukin-1 beta; IL-6, Interleukin-6; MAC, medial arterial calcification.
    Figure Legend Snippet: Pro-inflammatory milieu in calcified arteries and the impact of guide wires with different diameters on MAC model generation. (A) Representative IF staining results showed that CD68 (green color) and CD86 (red color) expression was low in the mice from the sham operation group and significantly higher in the mice from the experimental group in terms of the fluorescence density and intensity. Individual channels are presented separately for clarity. Nuclei were counterstained with DAPI (not shown). (B) Representative IHC staining results showed that the IL-1β and IL-6 expression in the calcified blood vessel from the experimental group was significantly higher than that in the sham operation group, with slightly higher intensity. (C) Incidence rates of ossification caused by the 0.35, 0.40, and 0.45 mm diameter guide wires. (D) According to the MAC histopathological grading criteria , the MAC grading ratios for the MAC caused by the 0.35, 0.40, and 0.45 mm diameter guide wires in the mouse MAC. Sample sizes were: n=10 for Sham group, n=12 for 0.35 mm group, and n=13 for 0.40 mm group, n=15 for 0.45 mm group. (E) Image of rough guide wires with diameters of 0.35, 0.40, or 0.45 mm. DAPI, 4',6-diamidino-2-phenylindole; IF, immunofluorescent; IHC, immunohistochemistry; IL-1β, Interleukin-1 beta; IL-6, Interleukin-6; MAC, medial arterial calcification.

    Techniques Used: Staining, Expressing, Fluorescence, Immunohistochemistry



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    Image Search Results


    Immunohistochemical detection of CD163 + and CD86 + TAMs in colorectal tissues (×200). (A) CD163 staining in various tissues. (B) CD86 staining. (C) IOD for CD163. (D) IOD for CD86. Data expressed as M (Q1, Q3) (n = 109). * P < 0.05, ** P < 0.01, *** P < 0.001 (DB-adjusted). Groups: 1, Normal; 2, CAS; 3, SSA; 4, CRC. M: Median; Q 1 : 1st Quartile; Q 3 : 3rd Quartile.

    Journal: Frontiers in Oncology

    Article Title: Tumor-associated macrophage expression in colorectal adenomas and carcinomas: relationship to Helicobacter pylori infection

    doi: 10.3389/fonc.2025.1649619

    Figure Lengend Snippet: Immunohistochemical detection of CD163 + and CD86 + TAMs in colorectal tissues (×200). (A) CD163 staining in various tissues. (B) CD86 staining. (C) IOD for CD163. (D) IOD for CD86. Data expressed as M (Q1, Q3) (n = 109). * P < 0.05, ** P < 0.01, *** P < 0.001 (DB-adjusted). Groups: 1, Normal; 2, CAS; 3, SSA; 4, CRC. M: Median; Q 1 : 1st Quartile; Q 3 : 3rd Quartile.

    Article Snippet: The following reagents and equipment were used in this study: mouse monoclonal anti-CD163 antibody (10D6, ma5-11458, Invitrogen, Waltham, MA02451, USA); rabbit monoclonal anti-CD86 antibody (EP1158-37, ab269587, Abcam, Cambridge, UK); rabbit Polyclonal a nti-CD68 antibody ( GB113150 , Servicebio, Wuhan, China); rabbit Polyclonal anti-CD163 antibody ( GB113152 , Servicebio, Wuhan, China); rabbit Polyclonal anti-CD86 antibody ( GB115630 , Servicebio, Wuhan, China); secondary antibodies and DAB chromogenic kits (Biomiky, Biosharp, and Servicebio, respectively).

    Techniques: Immunohistochemical staining, Staining

    Correlation of CD163 + /CD86 + TAMs levels with CRC development. (A) Differential expression between H.pylori -infected and uninfected groups. (B) Correlation between CD163 + and CD86 + expression. (C, D) Positive correlation of both markers with malignancy grade. Groups: 1, Normal; 2, CAS; 3, SSA; 4, CRC. *** P < 0.001 (MWU test).

    Journal: Frontiers in Oncology

    Article Title: Tumor-associated macrophage expression in colorectal adenomas and carcinomas: relationship to Helicobacter pylori infection

    doi: 10.3389/fonc.2025.1649619

    Figure Lengend Snippet: Correlation of CD163 + /CD86 + TAMs levels with CRC development. (A) Differential expression between H.pylori -infected and uninfected groups. (B) Correlation between CD163 + and CD86 + expression. (C, D) Positive correlation of both markers with malignancy grade. Groups: 1, Normal; 2, CAS; 3, SSA; 4, CRC. *** P < 0.001 (MWU test).

    Article Snippet: The following reagents and equipment were used in this study: mouse monoclonal anti-CD163 antibody (10D6, ma5-11458, Invitrogen, Waltham, MA02451, USA); rabbit monoclonal anti-CD86 antibody (EP1158-37, ab269587, Abcam, Cambridge, UK); rabbit Polyclonal a nti-CD68 antibody ( GB113150 , Servicebio, Wuhan, China); rabbit Polyclonal anti-CD163 antibody ( GB113152 , Servicebio, Wuhan, China); rabbit Polyclonal anti-CD86 antibody ( GB115630 , Servicebio, Wuhan, China); secondary antibodies and DAB chromogenic kits (Biomiky, Biosharp, and Servicebio, respectively).

    Techniques: Quantitative Proteomics, Infection, Expressing

    Multiplex immunofluorescence (200×) : CD68 + (red), CD163 + (green), CD86 + (green), DAPI (nuclei, blue), Merge (multichannel overlay), Scale bar: 50 μm. (A) CD68 + CD163 + dual-positive cells (IF). (B) CD68 + CD86 + dual-positive cells (IF).

    Journal: Frontiers in Oncology

    Article Title: Tumor-associated macrophage expression in colorectal adenomas and carcinomas: relationship to Helicobacter pylori infection

    doi: 10.3389/fonc.2025.1649619

    Figure Lengend Snippet: Multiplex immunofluorescence (200×) : CD68 + (red), CD163 + (green), CD86 + (green), DAPI (nuclei, blue), Merge (multichannel overlay), Scale bar: 50 μm. (A) CD68 + CD163 + dual-positive cells (IF). (B) CD68 + CD86 + dual-positive cells (IF).

    Article Snippet: The following reagents and equipment were used in this study: mouse monoclonal anti-CD163 antibody (10D6, ma5-11458, Invitrogen, Waltham, MA02451, USA); rabbit monoclonal anti-CD86 antibody (EP1158-37, ab269587, Abcam, Cambridge, UK); rabbit Polyclonal a nti-CD68 antibody ( GB113150 , Servicebio, Wuhan, China); rabbit Polyclonal anti-CD163 antibody ( GB113152 , Servicebio, Wuhan, China); rabbit Polyclonal anti-CD86 antibody ( GB115630 , Servicebio, Wuhan, China); secondary antibodies and DAB chromogenic kits (Biomiky, Biosharp, and Servicebio, respectively).

    Techniques: Multiplex Assay, Immunofluorescence

    Changes in cell density and proportion of CD68 + CD163 +/ CD68 + CD86 + dual-positive TAMs across groups. Groups: 1, Normal; 2, CRA; 3, CRC. *** P < 0.001 (Tukey HSD).

    Journal: Frontiers in Oncology

    Article Title: Tumor-associated macrophage expression in colorectal adenomas and carcinomas: relationship to Helicobacter pylori infection

    doi: 10.3389/fonc.2025.1649619

    Figure Lengend Snippet: Changes in cell density and proportion of CD68 + CD163 +/ CD68 + CD86 + dual-positive TAMs across groups. Groups: 1, Normal; 2, CRA; 3, CRC. *** P < 0.001 (Tukey HSD).

    Article Snippet: The following reagents and equipment were used in this study: mouse monoclonal anti-CD163 antibody (10D6, ma5-11458, Invitrogen, Waltham, MA02451, USA); rabbit monoclonal anti-CD86 antibody (EP1158-37, ab269587, Abcam, Cambridge, UK); rabbit Polyclonal a nti-CD68 antibody ( GB113150 , Servicebio, Wuhan, China); rabbit Polyclonal anti-CD163 antibody ( GB113152 , Servicebio, Wuhan, China); rabbit Polyclonal anti-CD86 antibody ( GB115630 , Servicebio, Wuhan, China); secondary antibodies and DAB chromogenic kits (Biomiky, Biosharp, and Servicebio, respectively).

    Techniques:

    Pro-inflammatory milieu in calcified arteries and the impact of guide wires with different diameters on MAC model generation. (A) Representative IF staining results showed that CD68 (green color) and CD86 (red color) expression was low in the mice from the sham operation group and significantly higher in the mice from the experimental group in terms of the fluorescence density and intensity. Individual channels are presented separately for clarity. Nuclei were counterstained with DAPI (not shown). (B) Representative IHC staining results showed that the IL-1β and IL-6 expression in the calcified blood vessel from the experimental group was significantly higher than that in the sham operation group, with slightly higher intensity. (C) Incidence rates of ossification caused by the 0.35, 0.40, and 0.45 mm diameter guide wires. (D) According to the MAC histopathological grading criteria , the MAC grading ratios for the MAC caused by the 0.35, 0.40, and 0.45 mm diameter guide wires in the mouse MAC. Sample sizes were: n=10 for Sham group, n=12 for 0.35 mm group, and n=13 for 0.40 mm group, n=15 for 0.45 mm group. (E) Image of rough guide wires with diameters of 0.35, 0.40, or 0.45 mm. DAPI, 4',6-diamidino-2-phenylindole; IF, immunofluorescent; IHC, immunohistochemistry; IL-1β, Interleukin-1 beta; IL-6, Interleukin-6; MAC, medial arterial calcification.

    Journal: Cardiovascular Diagnosis and Therapy

    Article Title: Establishment of a medial arterial calcification model in C57BL/6J mice via arterial intimal injury

    doi: 10.21037/cdt-2025-435

    Figure Lengend Snippet: Pro-inflammatory milieu in calcified arteries and the impact of guide wires with different diameters on MAC model generation. (A) Representative IF staining results showed that CD68 (green color) and CD86 (red color) expression was low in the mice from the sham operation group and significantly higher in the mice from the experimental group in terms of the fluorescence density and intensity. Individual channels are presented separately for clarity. Nuclei were counterstained with DAPI (not shown). (B) Representative IHC staining results showed that the IL-1β and IL-6 expression in the calcified blood vessel from the experimental group was significantly higher than that in the sham operation group, with slightly higher intensity. (C) Incidence rates of ossification caused by the 0.35, 0.40, and 0.45 mm diameter guide wires. (D) According to the MAC histopathological grading criteria , the MAC grading ratios for the MAC caused by the 0.35, 0.40, and 0.45 mm diameter guide wires in the mouse MAC. Sample sizes were: n=10 for Sham group, n=12 for 0.35 mm group, and n=13 for 0.40 mm group, n=15 for 0.45 mm group. (E) Image of rough guide wires with diameters of 0.35, 0.40, or 0.45 mm. DAPI, 4',6-diamidino-2-phenylindole; IF, immunofluorescent; IHC, immunohistochemistry; IL-1β, Interleukin-1 beta; IL-6, Interleukin-6; MAC, medial arterial calcification.

    Article Snippet: Subsequently, samples were incubated with rabbit anti-CD86 polyclonal antibody (1:100; Cat #19589; Cell Signaling Technology, Danvers, USA) diluted in 1% BSA/PBS at 4 °C overnight.

    Techniques: Staining, Expressing, Fluorescence, Immunohistochemistry